PBK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84342

Novus Biologicals, part of Bio-Techne
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NBP1-84342
NBP1-84342-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PBK Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PBK Antibody

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342]

Immunohistochemistry-Paraffin: PBK Antibody [NBP1-84342] - Staining of human colon, kidney, liver and testis using Anti-PBK antibody NBP1-84342 (A) shows similar protein distribution across tissues to independent antibody NBP2-58043 (B).
Western Blot: PBK Antibody [NBP1-84342]

Western Blot: PBK Antibody [NBP1-84342]

Western Blot: PBK Antibody [NBP1-84342] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: PBK Antibody [NBP1-84342]

Immunocytochemistry/ Immunofluorescence: PBK Antibody [NBP1-84342]

Immunocytochemistry/Immunofluorescence: PBK Antibody [NBP1-84342] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
View 9 more images

Applications for PBK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PBK

PBK encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.

Alternate Names

Cancer/testis antigen 84, CT84MAPKK-like protein kinase, EC 2.7.12.2, FLJ14385, Nori-3PDZ-binding kinase, PDZ binding kinase, serine/threonine protein kinase, Spermatogenesis-related protein kinase, SPKT-LAK cell-originated protein kinase, TOPKlymphokine-activated killer T-cell-originated protein kinase

Gene Symbol

PBK

Additional PBK Products

Product Documents for PBK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PBK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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