Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PCCA Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 678 to 728 of human Propionyl CoA Carboxylase, Alpha Polypeptide using the numbering given in entry NP_000273.2 (GeneID 5095).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCCA Antibody

Western Blot: PCCA Antibody [NBP2-32215]

Western Blot: PCCA Antibody [NBP2-32215]

Western Blot: PCCA Antibody [NBP2-32215] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PCCA antibody NBP2-32215 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-32215]

Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-32215]

Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-32215] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- PCCA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-32215]

Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-32215]

Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-32215] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PCCA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Applications for PCCA Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PCCA

PCCA is encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

EC 6.4.1, EC 6.4.1.3, pccA complementation group, PCCase alpha subunit, PCCase subunit alpha, propanoyl-CoA:carbon dioxide ligase alpha subunit, Propanoyl-CoA:carbon dioxide ligase subunit alpha, propionyl CoA carboxylase, alpha polypeptide, propionyl Coenzyme A carboxylase, alpha polypeptide, propionyl-CoA carboxylase alpha chain, mitochondrial

Gene Symbol

PCCA

UniProt

NP_000273.2 (Human)

Additional PCCA Products

Product Documents for PCCA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCCA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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