PCNA [p Tyr211] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-59861

Novus Biologicals, part of Bio-Techne
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NB100-59861
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.05 mg/ml

View all PCNA Antibodies »

Product Specifications

Immunogen

Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human proliferating cell nuclear antigen (GeneID 5111) around tyrosine 211 according to the numbering given inentry NP_002583.1.

Modification

p Tyr211

Marker

Proliferation Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCNA [p Tyr211] Antibody

Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861]

Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861]

Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast tissue. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used at at dilution of 1:250 and incubated for 18 hours at room temperature. Detection: DAB
Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861]

Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861]

Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used a dilution of 1:250 and incubated for 18 hours at room temeprature. Detection: DAB

Applications for PCNA [p Tyr211] Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500
Application Notes
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Extended incubation with primary antibody (16-24 hours) at room temperature is recommended. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.05 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PCNA

PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Entrez Gene IDs

5111 (Human)

Gene Symbol

PCNA

UniProt

P12004 (Human)

Additional PCNA Products

Product Documents for PCNA [p Tyr211] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PCNA [p Tyr211] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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