PIN/DLC8 Antibody (SD08-04)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67796

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67796
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SD08-04

Concentration

1 mg/ml

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Product Specifications

Immunogen

Synthetic peptide within Human PIN/DLC8 aa 1-50 / 89. (SwissProt: P63167 Human; SwissProt: P63168 Mouse; SwissProt: P63170 Rat)

Localization

Mitochondrion, Nucleus, centrosome, cytoskeleton.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIN/DLC8 Antibody (SD08-04)

Western Blot: PIN/DLC8 Antibody (SD08-04) [NBP2-67796]

Western Blot: PIN/DLC8 Antibody (SD08-04) [NBP2-67796]

Western Blot: PIN/DLC8 Antibody (SD08-04) [NBP2-67796] - Analysis of DYNLL1 on MCF-7 cells lysates using anti-DYNLL1 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: PIN/DLC8 Antibody (SD08-04) [NBP2-67796]

Immunocytochemistry/ Immunofluorescence: PIN/DLC8 Antibody (SD08-04) [NBP2-67796]

Immunocytochemistry/Immunofluorescence: PIN/DLC8 Antibody (SD08-04) [NBP2-67796] - Staining DYNLL1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PIN/DLC8 Antibody (SD08-04) [NBP2-67796]

Immunohistochemistry-Paraffin: PIN/DLC8 Antibody (SD08-04) [NBP2-67796]

Immunohistochemistry-Paraffin: PIN/DLC8 Antibody (SD08-04) [NBP2-67796] - Analysis of paraffin-embedded rat esophagus tissue using anti-DYNLL1 antibody. Counter stained with hematoxylin.
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Applications for PIN/DLC8 Antibody (SD08-04)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIN

DLC8 protein bound efficiently to gephyrin in in vitro binding assays and colocalized with gephyrin during coexpression in HEK293 cells. The binding site for DLC8 was mapped to a fragment of 63 amino acids within the central linker domain of gephyrin. In hippocampal neurons, endogenous DLC8 protein was enriched at synaptic sites identified by synaptophysin and gephyrin immunostaining. Because DLC8 has been described as stoichiometric components of cytoplasmic dynein and myosin-Va complexes, results suggest that motor proteins are involved in the subcellular localization of gephyrin. DLC8 was identified as a transport molecule in the cytoplasm. DLC8 co-localizes with TRPS1 in dot-like structures in the cell nucleus. In an electrophoretic mobility shift assay it was shown that the interaction of DLC8 and TRPS1 lowers the binding of TRPS1 to the GATA consensus sequence. In addition DLC8 is able to suppress the transcriptional repression activity of TRPS1.

Long Name

Protein Inhibitor of Neuronal Nitric Oxide Synthase

Alternate Names

DLC1, DLC8, DNCL1, DNCLC1, DYNLL1, LC8a

Gene Symbol

DYNLL1

Additional PIN Products

Product Documents for PIN/DLC8 Antibody (SD08-04)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PIN/DLC8 Antibody (SD08-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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