PINK1 Antibody (8E10.1D6) - BSA Free

Western Blot: PINK1 Antibody (8E10.1D6)BSA Free [NBP2-36488]

Western Blot: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Whole cell protein from HeLa cells treated with or without valinomycin (1 uM, 24h) as indicated was separated by SDS-PAGE on a 7.5% polyacrylamide gel. Protein was transferred to PVDF membrane and probed with 2 ug/ml anti-PINK1 in 1% BSA and detected with an HRP-conjugated anti-mouse secondary antibody using chemiluminescence. PINK1 is seen to be upregulated with treament and with a molecular weight at approximately 60 kDa.

Western Blot: PINK1 Antibody (8E10.1D6)BSA Free [NBP2-36488]

Western Blot: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Analysis of (A) Partial Recombinant Human PINK-1 protein with estimated molecular weight at 13kDa and (B) Human Liver lysate using PINK1 antibody clone 8E10.1D6 at 3ug/ml concentration, molecular weight ~64 kDa.

Western Blot: PINK1 Antibody (8E10.1D6) - BSA Free [NBP2-36488] -

Western Blot: PINK1 Antibody (8E10.1D6) - BSA Free [NBP2-36488] - Active protein synthesis is required for CMR-induced cytoplasmic vacuolation. Analysis of CMR-induced cytoplasmic vacuolation in MDA-MB-231 & PC-3 cells by (a) immunocytochemistry & (b) Western blot analysis. Cropped blots were displayed, & original images were included in Fig. S24. The effect of cycloheximide (CHX) on (c) cell viability & (d) cell death was detected by MTT & PI uptake assays. Cells were pre-treated with 5 μg/ml CHX for 1 h. All cells were treated with 6 μM CMR for 48 h. Red: Calreticulin. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29934599), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PINK1 Antibody (8E10.1D6) - BSA Free [NBP2-36488] -

Western Blot: PINK1 Antibody (8E10.1D6) - BSA Free [NBP2-36488] - PINK1 is responsible for CMR-induced cytoplasmic vacuolation. (a) Analysis of the effect of ectopic expression of Myc-PINK1 on CMR-induced expression levels of Alix & cytoplasmic vacuolation in LNCaP cells by Western blot (left panel), phase-contrast (middle panel) & immunocytochemistry (right panel). (b) The effect of ectopic expression of Myc-PINK1 on the expression levels of Alix in MDA-MB-231 cells as measured by Western blot. (c) The effect of siRNA-mediated knockdown of PINK1 on CMR-induced changes in the expression of Alix in MDA-MB-231 cells as analyzed by Western blot (left panel) & immunocytochemistry (right panel). Red: Calreticulin. All cells were treated with 6 μM CMR for 48 h. Cropped blots were displayed, & original images were included in Figs S21–S23. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29934599), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PINK1 Antibody (8E10.1D6) - BSA Free [NBP2-36488] -

Western Blot: PINK1 Antibody (8E10.1D6) - BSA Free [NBP2-36488] - Regulation of the mitophagic marker PINK1 in WT & TLR4 KO mice. a PINK1 immunoreactivity was observed in the spinal dorsal horn of CCI mice. PINK1 IR cells were significantly increased in the ipsilateral compared with the contralateral side in WT mice, & no significant difference was found in TLR4 KO mice. Scale bar = 50 μm in A1, A3, A5, & A7. Scale bar = 20 μm in A2, A4, A6, & A8. b The number of PINK1 IR cells was significantly increased in the ipsilateral compared with the contralateral side of WT mice. c Expression of PINK1 was assessed by Western blotting. The PINK1 protein levels were significantly increased in the ipsilateral side compared with the contralateral side in WT mice, & no significant difference was shown in TLR4 KO mice. (D) Quantification by densitometry with Image J. Two-way ANOVA; all the data are shown as mean ± standard deviation, where *P < 0.05 denotes a significant difference compared with the control group. d Frozen sections (WT-CCI, POD7) were stained with PINK1 & co-stained with anti-GFAP (A1–4), anti-iba-1 (B1–4), & anti-NeuN antibodies (C1–4). A4, B4 & C4 is rectangular magnification of merged A3, B3 & C3, respectively. Scale bar = 50 μm in A, B, C1–3. Scale bar = 20 μm in A, B, C4 Image collected & cropped by CiteAb from the following publication (https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-018-0354-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.