PIP5K1 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19833

Novus Biologicals, part of Bio-Techne
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NBP2-19833
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%), Canine (95%), Chimpanzee (99%), Porcine (96%), Rat (91%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PIP5K1 alpha Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PIP5K1 alpha. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%), Chicken (85%).

Localization

Cell membrane; Endomembrane system; Golgi apparatus; Golgi stack (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIP5K1 alpha Antibody

Western Blot: PIP5K1 alpha Antibody [NBP2-19833]

Western Blot: PIP5K1 alpha Antibody [NBP2-19833]

Western Blot: PIP5K1 alpha Antibody [NBP2-19833] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PIP5K1 alpha Antibody [NBP2-19833]

Immunocytochemistry/ Immunofluorescence: PIP5K1 alpha Antibody [NBP2-19833]

Immunocytochemistry/Immunofluorescence: PIP5K1 alpha Antibody [NBP2-19833] - Immunofluorescence analysis of methanol-fixed A549, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PIP5K1 alpha Antibody [NBP2-19833]

Immunohistochemistry-Paraffin: PIP5K1 alpha Antibody [NBP2-19833]

Immunohistochemistry-Paraffin: PIP5K1 alpha Antibody [NBP2-19833] - CL1-0 xenograft, using PIPK I alpha antibody at 1:100 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
View 1 more images

Applications for PIP5K1 alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PIP5K1 alpha

Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2

Alternate Names

68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha, EC 2.7.1, EC 2.7.1.68, Phosphatidylinositol-4-phosphate 5-kinase type I alpha, phosphatidylinositol-4-phosphate 5-kinase type-1 alpha, phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, PIP5K1-alpha, PIP5KIalpha, PtdIns(4)P-5-kinase 1 alpha

Gene Symbol

PIP5K1A

UniProt

Q99755 (Human)

Additional PIP5K1 alpha Products

Product Documents for PIP5K1 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PIP5K1 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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