PITPNM3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31070
![Knockdown Validated: PITPNM3 Antibody [NBP1-31070]](https://resources.bio-techne.com/images/products/PITPNM3-Antibody-Knockdown-Validated-NBP1-31070-img0005.jpg "Knockdown Validated: PITPNM3 Antibody [NBP1-31070]")
![Knockdown Validated: PITPNM3 Antibody [NBP1-31070]](https://resources.bio-techne.com/images/products/PITPNM3-Antibody-Knockdown-Validated-NBP1-31070-img0006.jpg "Knockdown Validated: PITPNM3 Antibody [NBP1-31070]")
![Western Blot: PITPNM3 Antibody [NBP1-31070]](https://resources.bio-techne.com/images/products/PITPNM3-Antibody-Western-Blot-NBP1-31070-img0004.jpg "Western Blot: PITPNM3 Antibody [NBP1-31070]")
Discontinued Product
NBP1-31070 has been discontinued.
View all PITPNM3 products.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PITPNM3. The exact sequence is proprietary.
Localization
Endomembrane system, Peripheral membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PITPNM3 Antibody
Western Blot: PITPNM3 Antibody [NBP1-31070]
Western Blot: PITPNM3 Antibody [NBP1-31070] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NIR1 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: PITPNM3 Antibody [NBP1-31070]](https://resources.bio-techne.com/images/products/PITPNM3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31070-img0007.jpg "Immunocytochemistry/ Immunofluorescence: PITPNM3 Antibody [NBP1-31070]")
Immunocytochemistry/ Immunofluorescence: PITPNM3 Antibody [NBP1-31070]
Immunocytochemistry/Immunofluorescence: PITPNM3 Antibody [NBP1-31070] - Representative fluorescence microscopy images of CCL18 binding to naive CD4+ T cells, knocked down or not for PITPNM3, stained for PITPNM3 and CCL18 3 h after adding CCL18. Scale bar, 5 um. Shown are the representative images for three independent experiments using PB T cells from three normal donors. Image collected and cropped by CiteAb from the following publication (nature.com/articles/cr201734), licensed under a CC-BY license.![Immunocytochemistry/ Immunofluorescence: PITPNM3 Antibody [NBP1-31070]](https://resources.bio-techne.com/images/products/PITPNM3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31070-img0002.jpg "Immunocytochemistry/ Immunofluorescence: PITPNM3 Antibody [NBP1-31070]")
Immunocytochemistry/ Immunofluorescence: PITPNM3 Antibody [NBP1-31070]
Immunocytochemistry/Immunofluorescence: PITPNM3 Antibody [NBP1-31070] - Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: PITPNM3 protein stained by NIR1 antibody, C-term diluted at 1:500. Blue: Hoechst 33343 staining.Applications for PITPNM3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Application Notes
Knockdown validation (Jiang et al).
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PITPNM3
Alternate Names
membrane-associated phosphatidylinositol transfer protein 3, MGC157740, MGC157741, NIR-1, NIR1CORD5, Phosphatidylinositol transfer protein, membrane-associated 3, PITPnm 3, PITPNM family member 3, Pyk2 N-terminal domain-interacting receptor 1, RDGBA3, retinal degeneration B alpha 3
Gene Symbol
PITPNM3
UniProt
Additional PITPNM3 Products
Product Documents for PITPNM3 Antibody
Product Specific Notices for PITPNM3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.