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PPP4R1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87242

Novus Biologicals, part of Bio-Techne
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NBP1-87242

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPEVGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPP4R1 Antibody

Western Blot: PPP4R1 Antibody [NBP1-87242]

Western Blot: PPP4R1 Antibody [NBP1-87242]

Western Blot: PPP4R1 Antibody [NBP1-87242] - Analysis using Anti-PPP4R1 antibody NBP1-87242 (A) shows similar pattern to independent antibody NBP1-87241 (B).
Immunocytochemistry/ Immunofluorescence: PPP4R1 Antibody [NBP1-87242]

Immunocytochemistry/ Immunofluorescence: PPP4R1 Antibody [NBP1-87242]

Immunocytochemistry/Immunofluorescence: PPP4R1 Antibody [NBP1-87242] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PPP4R1 Antibody [NBP1-87242]

Immunohistochemistry-Paraffin: PPP4R1 Antibody [NBP1-87242]

Immunohistochemistry-Paraffin: PPP4R1 Antibody [NBP1-87242] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.

Applications for PPP4R1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPP4R1

Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3

Alternate Names

MEG1, PP4R1PP4(Rmeg), protein phosphatase 4, regulatory subunit 1, serine/threonine phosphatase 4, serine/threonine-protein phosphatase 4 regulatory subunit 1

Gene Symbol

PPP4R1

Additional PPP4R1 Products

Product Documents for PPP4R1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPP4R1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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