pre-mRNA cleavage factor I (59 kDa subunit) Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89868
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for pre-mRNA cleavage factor I (59 kDa subunit) Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SDDRSSSTEPPPPVRQEPSPKPNNKTPAILYTYSGLRNRRAAVYVGSFSWWTTDQQLIQVIRSIGVYDVVELKFAENRA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for pre-mRNA cleavage factor I (59 kDa subunit) Antibody
Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868]
Western Blot: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/ Immunofluorescence: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868]
Immunocytochemistry/Immunofluorescence: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868]
Immunohistochemistry-Paraffin: pre-mRNA cleavage factor I (59 kDa subunit) Antibody [NBP1-89868] - Staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.Applications for pre-mRNA cleavage factor I (59 kDa subunit) Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: pre-mRNA cleavage factor I (59 kDa subunit)
Alternate Names
CFIm59, cleavage and polyadenylation specific factor 7, 59kDa, CPSF 59 kDa subunit, FLJ12529,59 kDa subunit, FLJ39024, MGC9315, pre-mRNA cleavage factor I, 59 kDa subunit
Gene Symbol
CPSF7
Additional pre-mRNA cleavage factor I (59 kDa subunit) Products
Product Documents for pre-mRNA cleavage factor I (59 kDa subunit) Antibody
Product Specific Notices for pre-mRNA cleavage factor I (59 kDa subunit) Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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