Prothrombin Antibody (S08-5G1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20051

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-20051-100ul
NBP3-20051-50ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-5G1

Concentration

0.3 mg/ml

View all Prothrombin Antibodies »

Product Specifications

Immunogen

Recombinant protein of mouse Prothrombin (Uniprot # P19221)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Prothrombin Antibody (S08-5G1)

Western Blot: Prothrombin Antibody (S08-5G1) [NBP3-20051]

Western Blot: Prothrombin Antibody (S08-5G1) [NBP3-20051]

Western Blot: Prothrombin Antibody (S08-5G1) [NBP3-20051] - Western blot detection of Prothrombin in mouse liver, rat liver cell lysates using NBP3-20051 (1:1000 diluted). Predicted band size: 38kDa. Observed band size: 45kDa.

Applications for Prothrombin Antibody (S08-5G1)

Application
Recommended Usage

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Prothrombin

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) at residue 44 leads to formation of Thrombin in the first step of the coagulation cascade. Thrombin cleaves bonds between Arg and Gly and activates Factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.

Alternate Names

Coagulation factor II, coagulation factor II (thrombin), EC 3.4.21, EC 3.4.21.5, prothrombin, prothrombin B-chain, PT, serine protease

Gene Symbol

F2

Additional Prothrombin Products

Product Documents for Prothrombin Antibody (S08-5G1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Prothrombin Antibody (S08-5G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.