PURL Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84691
![Immunocytochemistry/ Immunofluorescence: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84691-img0009.jpg "Immunocytochemistry/ Immunofluorescence: PURL Antibody [NBP1-84691]")
![Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Immunohistochemistry-Paraffin-NBP1-84691-img0014.jpg "Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]")
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHEELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PURL Antibody
![Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Immunohistochemistry-Paraffin-NBP1-84691-img0020.jpg "Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]")
Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]
Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691] - Staining of human kidney, lymph node, skeletal muscle and testis using Anti-PFAS antibody NBP1-84691 (A) shows similar protein distribution across tissues to independent antibody NBP1-84692 (B).![Western Blot: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Western-Blot-NBP1-84691-img0024.jpg "Western Blot: PURL Antibody [NBP1-84691]")
Western Blot: PURL Antibody [NBP1-84691]
Western Blot: PURL Antibody [NBP1-84691] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.![Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Immunohistochemistry-Paraffin-NBP1-84691-img0023.jpg "Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]")
![Western Blot: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Western-Blot-NBP1-84691-img0007.jpg "Western Blot: PURL Antibody [NBP1-84691]")
![Western Blot: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Western-Blot-NBP1-84691-img0019.jpg "Western Blot: PURL Antibody [NBP1-84691]")
![Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Immunohistochemistry-Paraffin-NBP1-84691-img0011.jpg "Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]")
![Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Immunohistochemistry-Paraffin-NBP1-84691-img0013.jpg "Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]")
![Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Immunohistochemistry-Paraffin-NBP1-84691-img0017.jpg "Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]")
![Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Immunohistochemistry-Paraffin-NBP1-84691-img0018.jpg "Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]")
![Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]](https://resources.bio-techne.com/images/products/PURL-Antibody-Immunohistochemistry-Paraffin-NBP1-84691-img0022.jpg "Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691]")
Applications for PURL Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20-1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PURL
Alternate Names
FGAM synthase, FGAMS, FGAR amidotransferase, formylglycinamide ribotide synthetase, KIAA0361, phosphoribosylformylglycinamidine synthase, PURL
Gene Symbol
PFAS
Additional PURL Products
Product Documents for PURL Antibody
Product Specific Notices for PURL Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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