ROCK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86124

Novus Biologicals, part of Bio-Techne
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NBP1-86124
NBP1-86124-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ROCK2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADK

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23319410)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROCK2 Antibody

Western Blot: ROCK2 Antibody [NBP1-86124]

Western Blot: ROCK2 Antibody [NBP1-86124]

Western Blot: ROCK2 Antibody [NBP1-86124] - Analysis in human cell line RH-30.
Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124]

Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124]

Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124] - Staining of human liver shows no positivity in hepatocytes as expected.
Western Blot: ROCK2 Antibody [NBP1-86124]

Western Blot: ROCK2 Antibody [NBP1-86124]

Western Blot: ROCK2 Antibody [NBP1-86124] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
View 3 more images

Applications for ROCK2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 26330114). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROCK2

ROCK2 is encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Long Name

Rho-associated, Coiled-coil Containing Protein Kinase 2

Alternate Names

ROCK-II, ROKalpha

Gene Symbol

ROCK2

Additional ROCK2 Products

Product Documents for ROCK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ROCK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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