Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RPS11 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 108 and 158 of human Ribosomal Protein S11 using the numbering given in entry NP_001006.1 (GeneID 6205).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPS11 Antibody

Western Blot: RPS11 Antibody [NBP2-22289]

Western Blot: RPS11 Antibody [NBP2-22289]

Western Blot: RPS11 Antibody [NBP2-22289] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: RPS11 Antibody [NBP2-22289]

Immunohistochemistry-Paraffin: RPS11 Antibody [NBP2-22289]

Immunohistochemistry-Paraffin: RPS11 Antibody [NBP2-22289] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- RPS11 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: RPS11 Antibody [NBP2-22289]

Immunoprecipitation: RPS11 Antibody [NBP2-22289]

Immunoprecipitation: RPS11 Antibody [NBP2-22289] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RPS11 antibody NBP2-22289 used for IP at 6 ug/mg lysate. RPS11 was also immunoprecipitated by rabbit anti-RPS11 antibody NBP2-22288. For blotting immunoprecipitated RPS11, NBP2-22289 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for RPS11 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPS11

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S17) is thought to be involved in the recognition of termination codons. This gene is co-transcribed with a small nucleolar RNA gene, which is located in its third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (provided by RefSeq)

Alternate Names

ribosomal protein S11,40S ribosomal protein S11

Gene Symbol

RPS11

UniProt

NP_001006.1 (Human)

Additional RPS11 Products

Product Documents for RPS11 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPS11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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