Siglec-9 Antibody (191240) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB1139CL1
Clone 191240 was used by HLDA to establish CD designation
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow Cytometry, Neutralization, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2A Clone # 191240
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-9
Gln18-Gly348 (predicted)
Accession # Q9Y336
Gln18-Gly348 (predicted)
Accession # Q9Y336
Specificity
Detects human Siglec-9 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouseSiglec‑E is observed and less than 1% cross-reactivity with recombinant human (rh) Siglec-3, rhSiglec-5, rhSiglec-6, rhSiglec-7, rhSiglec-8 or rhSiglec-10 is observed. In Western blots, approximately 100% cross-reactivity with recombinant mouse Siglec‑E is observed undernon-reduced conditions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Siglec-9 Antibody (191240) [CoraFluor™ 1]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
ELISA Capture (Matched Antibody Pair)
Optimal dilutions of this antibody should be experimentally determined.
ELISA Detection (Matched Antibody Pair)
Optimal dilutions of this antibody should be experimentally determined.
ELISA Standard (Matched Pair)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Siglec-9
Long Name
Sialic Acid Binding Ig-like Lectin 9
Alternate Names
CD329, OBBP-Like, Siglec9
Gene Symbol
SIGLEC9
Additional Siglec-9 Products
Product Documents for Siglec-9 Antibody (191240) [CoraFluor™ 1]
Product Specific Notices for Siglec-9 Antibody (191240) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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