SPIRE1 Antibody (4C5)
Novus Biologicals, part of Bio-Techne | Catalog # H00056907-M01
Key Product Details
Species Reactivity
Validated:
Human, Hamster
Cited:
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4C5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SPIRE1 (NP_064533, 482 a.a. ~ 583 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SEKPSTAHHRPLRSIARFSSKSKSMDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTISEI
Reactivity Notes
Human. Other species not tested.
Specificity
SPIRE1 - spire homolog 1 (Drosophila)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for SPIRE1 Antibody (4C5)
![Western Blot: SPIRE1 Antibody (4C5) [H00056907-M01]](https://resources.bio-techne.com/images/products/SPIRE1-Antibody-4C5-Western-Blot-H00056907-M01-img0003.jpg "Western Blot: SPIRE1 Antibody (4C5) [H00056907-M01]")
Western Blot: SPIRE1 Antibody (4C5) [H00056907-M01]
Western Blot: SPIRE1 Antibody (4C5) [H00056907-M01] - Analysis of SPIRE1 expression in transfected 293T cell line by SPIRE1 monoclonal antibody (M01), clone 4C5.Lane 1: SPIRE1 transfected lysate(15 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: SPIRE1 Antibody (4C5) [H00056907-M01] - Detection limit for recombinant GST tagged SPIRE1 is approximately 0.03ng/ml as a capture antibody.
Applications for SPIRE1 Antibody (4C5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SPIRE1
Alternate Names
KIAA1135, MGC150621, MGC150622, protein spire homolog 1, SPIR1, Spir-1, spire homolog 1 (Drosophila)
Entrez Gene IDs
56907 (Human)
Gene Symbol
SPIRE1
OMIM
609216 (Human)
UniProt
Additional SPIRE1 Products
Product Documents for SPIRE1 Antibody (4C5)
Product Specific Notices for SPIRE1 Antibody (4C5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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