SPIRE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92438

Novus Biologicals, part of Bio-Techne
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NBP1-92438
NBP1-92438-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPIRE1 Antibodies »

Product Summary for SPIRE1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LEEIKAERKLRPVSPEEIRRSRLDVTTPESTKNLVESSMVNGGLTSQTKENGLSTSQQVPAQRKKLLRAPTLAELDSSESEEETLHK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPIRE1 Antibody

Western Blot: SPIRE1 Antibody [NBP1-92438]

Western Blot: SPIRE1 Antibody [NBP1-92438]

Western Blot: SPIRE1 Antibody [NBP1-92438] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: SPIRE1 Antibody [NBP1-92438]

Immunocytochemistry/ Immunofluorescence: SPIRE1 Antibody [NBP1-92438]

Immunocytochemistry/Immunofluorescence: SPIRE1 Antibody [NBP1-92438] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: SPIRE1 Antibody [NBP1-92438]

Immunohistochemistry-Paraffin: SPIRE1 Antibody [NBP1-92438]

Immunohistochemistry-Paraffin: SPIRE1 Antibody [NBP1-92438] - Staining of human lateral ventricle shows strong nuclear positivity in neuronal cells.

Applications for SPIRE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPIRE1

Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]).[supplied by OMIM]

Alternate Names

KIAA1135, MGC150621, MGC150622, protein spire homolog 1, SPIR1, Spir-1, spire homolog 1 (Drosophila)

Gene Symbol

SPIRE1

Additional SPIRE1 Products

Product Documents for SPIRE1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPIRE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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