Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Theoretical MW

146.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TBC1D4 Antibody (OTI5E6)

Western Blot: TBC1D4 Antibody (OTI5E6) [NBP2-00597]

Western Blot: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.

Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody (OTI5E6) [NBP2-00597]

Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody (OTI5E6) [NBP2-00597] - TBC1D4 Antibody (5E6) [NBP2-00597] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.

Flow Cytometry: TBC1D4 Antibody (OTI5E6) [NBP2-00597]

Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TBC1D4 antibody, and then analyzed by flow cytometry.

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Applications for TBC1D4 Antibody (OTI5E6)

Application

Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.35 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TBC1D4

AS160, also known as TBC1D4, is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS-160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of Thr642 is also important in mediating TBC1D4 redistribution from low density microsomes to the cytosol in adipocytes.

Alternate Names

Akt substrate of 160 kDa, AS160Acrg embryonic lethality minimal region ortholog, DKFZp779C0666, KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein, TBC1 domain family member 4, TBC1 domain family, member 4

Entrez Gene IDs

9882 (Human)

Gene Symbol

TBC1D4

Additional TBC1D4 Products

Product Documents for TBC1D4 Antibody (OTI5E6)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for TBC1D4 Antibody (OTI5E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

TBC1D4 Antibody (OTI5E6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00597

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Concentration

View all TBC1D4 Antibodies »

Product Summary for TBC1D4 Antibody (OTI5E6)

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for TBC1D4 Antibody (OTI5E6)

Western Blot: TBC1D4 Antibody (OTI5E6) [NBP2-00597]

Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody (OTI5E6) [NBP2-00597]

Flow Cytometry: TBC1D4 Antibody (OTI5E6) [NBP2-00597]

Flow Cytometry: TBC1D4 Antibody (OTI5E6) [NBP2-00597]

Flow Cytometry: TBC1D4 Antibody (OTI5E6) [NBP2-00597]

Applications for TBC1D4 Antibody (OTI5E6)

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: TBC1D4

Alternate Names

Entrez Gene IDs

Gene Symbol

Additional TBC1D4 Products

Product Documents for TBC1D4 Antibody (OTI5E6)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for TBC1D4 Antibody (OTI5E6)