TTF-1 / NKX2-1 Antibody (6C11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26111

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6C11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TTF-1/NKX2-1 Antibodies »

Product Summary for TTF-1 / NKX2-1 Antibody (6C11)

Immunogen

A synthesized peptide derived from Human TTF-1/NKX2-1 [UniProt P43699]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TTF-1 / NKX2-1 Antibody (6C11)

TTF-1 / NKX2-1 Antibody (6C11)

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] -

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] - Image of TTF-1 / NKX2-1 Antibody (6C11) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
TTF-1 / NKX2-1 Antibody (6C11)

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] -

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] - Image of TTF-1 / NKX2-1 Antibody (6C11) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
TTF-1 / NKX2-1 Antibody (6C11)

Western Blot: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] -

Western Blot: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] - Positive Western Blot detected in: HepG2 whole cell lysate, U251 whole cell lysate, U87 whole cell lysate.
All lanes: TTF-1 / NKX2-1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 39 kDa
Observed band size: 39 kDa
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Applications for TTF-1 / NKX2-1 Antibody (6C11)

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: TTF-1/NKX2-1

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Long Name

NK2 Homeobox 1

Alternate Names

BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1

Gene Symbol

NKX2-1

Additional TTF-1/NKX2-1 Products

Product Documents for TTF-1 / NKX2-1 Antibody (6C11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TTF-1 / NKX2-1 Antibody (6C11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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