UBE2E2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92556

Novus Biologicals, part of Bio-Techne
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NBP1-92556
NBP1-92556-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE2E2 Antibodies »

Product Summary for UBE2E2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: STEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE2E2 Antibody

Western Blot: UBE2E2 Antibody [NBP1-92556]

Western Blot: UBE2E2 Antibody [NBP1-92556]

Western Blot: UBE2E2 Antibody [NBP1-92556] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: UBE2E2 Antibody [NBP1-92556]

Immunocytochemistry/ Immunofluorescence: UBE2E2 Antibody [NBP1-92556]

Immunocytochemistry/Immunofluorescence: UBE2E2 Antibody [NBP1-92556] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: UBE2E2 Antibody [NBP1-92556]

Immunohistochemistry-Paraffin: UBE2E2 Antibody [NBP1-92556]

Immunohistochemistry-Paraffin: UBE2E2 Antibody [NBP1-92556] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
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Applications for UBE2E2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2E2

UBE2E2 catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins

Alternate Names

EC 6.3.2.19, FLJ25157, UbcH8, Ubiquitin carrier protein E2, ubiquitin-conjugating enzyme E2 E2, ubiquitin-conjugating enzyme E2E 2 (homologous to yeast UBC4/5), ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast), Ubiquitin-protein ligase E2

Gene Symbol

UBE2E2

Additional UBE2E2 Products

Product Documents for UBE2E2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2E2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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