UBE2R1/CDC34 Antibody (1P10A8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16882

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16882-100ul
NBP3-16882-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1P10A8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UBE2R1/CDC34 Antibodies »

Product Summary for UBE2R1/CDC34 Antibody (1P10A8)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-236 of human UBE2R1/CDC34 (P49427). LQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE2R1/CDC34 Antibody (1P10A8)

Western Blot: UBE2R1/CDC34 Antibody (1P10A8) [NBP3-16882]

Western Blot: UBE2R1/CDC34 Antibody (1P10A8) [NBP3-16882]

Western Blot: UBE2R1/CDC34 Antibody (1P10A8) [NBP3-16882] - Western blot analysis of extracts of various cell lines, using UBE2R1/CDC34 Rabbit mAb (NBP3-16882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for UBE2R1/CDC34 Antibody (1P10A8)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2R1/CDC34

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Alternate Names

Cdc34, UBC3

Gene Symbol

CDC34

Additional UBE2R1/CDC34 Products

Product Documents for UBE2R1/CDC34 Antibody (1P10A8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2R1/CDC34 Antibody (1P10A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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