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West Nile Virus MP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56743

Novus Biologicals, part of Bio-Techne
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NB100-56743SS
NB100-56743

Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Human, Mouse, Hamster, Primate - Macaca mulatta (Rhesus Macaque), Virus

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acid residues 8-27 (GESTLANKKGAWLDSTKATR) of West Nile Virus MP. Genbank accession no. NP_776013.

Reactivity Notes

West Nile Virus glycoprotein M (Genbank accession no. NP_776013).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for West Nile Virus MP Antibody - BSA Free

Western Blot: West Nile Virus MP AntibodyBSA Free [NB100-56743]

Western Blot: West Nile Virus MP AntibodyBSA Free [NB100-56743]

Western Blot: West Nile Virus MP Antibody - BSA Free [NB100-56743] - Analysis of West Nile Virus West Nile Virus MP in (A) untransfected and (B) transfected mouse melanoma cells using NB100-56743 at 1 ug/ml.
Immunohistochemistry-Paraffin: West Nile Virus MP Antibody - BSA Free [NB100-56743]

Immunohistochemistry-Paraffin: West Nile Virus MP Antibody - BSA Free [NB100-56743]

Immunohistochemistry-Paraffin: West Nile Virus MP Antibody - BSA Free [NB100-56743] - Analysis of West Nile Virus MP in bird liver using this antibody at 1 ug/ml, incubated for 30 minutes. A Ventana brand second step antibody conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. The antigen retrieval method used to obtain staining: Ventana Protease 2 for three minutes at room temperature with a Parafilm coverslip. (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute). Image and protocol used to generate this image are courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine.

Applications for West Nile Virus MP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:1000. Use reported in scientific literature (Tan et al (2009))

Immunohistochemistry

1:20-1:1000

Immunohistochemistry-Paraffin

1.0 ug/ ml

Immunoprecipitation

reported in scientific literature (Tan et al (2009))

Western Blot

0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: West Nile Virus MP

West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.

Additional West Nile Virus MP Products

Product Documents for West Nile Virus MP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for West Nile Virus MP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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