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WIPI1 Antibody (2F4G9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16835

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16835-100ul
NBP3-16835-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2F4G9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for WIPI1 Antibody (2F4G9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human WIPI1 (Q5MNZ9). MEAEAADAPPGGVESALSCFSFNQDCTSLATGTKAGYKLFSLSSVEQLDQVHGSNEIPDVYIVERLFSSSLVVVVSHTKPRQMNVYHFKKGTEICNYSYS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WIPI1 Antibody (2F4G9)

Western Blot: WIPI1 Antibody (2F4G9) [NBP3-16835]

Western Blot: WIPI1 Antibody (2F4G9) [NBP3-16835]

Western Blot: WIPI1 Antibody (2F4G9) [NBP3-16835] - Western blot analysis of extracts of various cell lines, using WIPI1 Rabbit mAb (NBP3-16835) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for WIPI1 Antibody (2F4G9)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WIPI1

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Alternate Names

Atg18, Atg18 protein homolog, ATG18A, FLJ10055, WD repeat domain phosphoinositide-interacting protein 1, WD repeat domain, phosphoinositide interacting 1, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WD40 repeat protein Interacting with phosphoInositides of 49kDa, WIPI 49 kDa, WIPI-1, WIPI-1 alpha, WIPI49ATG18

Gene Symbol

WIPI1

Additional WIPI1 Products

Product Documents for WIPI1 Antibody (2F4G9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WIPI1 Antibody (2F4G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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