ZNF423 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76351
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Summary for ZNF423 Antibody
Immunogen
Peptide with sequence EPECDQKTSRALEDR corresponding to N-Terminus (near) according to NP_055884.2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for ZNF423 Antibody
Immunocytochemistry/ Immunofluorescence: ZNF423 Antibody [NBP2-76351]
Immunocytochemistry/Immunofluorescence: ZNF423 Antibody [NBP2-76351] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Flow Cytometry: ZNF423 Antibody [NBP2-76351]
Flow Cytometry: ZNF423 Antibody [NBP2-76351] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for ZNF423 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:2000
Application Notes
WB: Preliminary experiments in human brain (cerebellum) lysates and in mouse and rat brain lysates gave no specific signal but low background.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ZNF423
Alternate Names
FLJ20086, KIAA1584, MGC21637, MGC61687, SUHW4, Suppressor of hairy wing homolog 4, zinc finger protein 280D, Zinc finger protein 634, ZNF634suppressor of hairy wing homolog 4 (Drosophila)
Gene Symbol
ZNF423
Additional ZNF423 Products
Product Specific Notices for ZNF423 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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