Proteome Profiler Human Adipokine Array Kit
R&D Systems, part of Bio-Techne | Catalog # ARY024
Key Product Details
Summary for Proteome Profiler Human Adipokine Array Kit
To simultaneously detect the relative levels of 58 different obesity-related molecules in a single sample. No specialized equipment is necessary.
- Troubleshooting Guide
- Assays for analytes represented in the Human Adipokine Array Kit
- Compatible with LI-COR* and chemiluminescence detection
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, plasma, saliva, urine, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8°.
| Simultaneously detect the levels of these obesity-related proteins in a single sample. | ||
| Adiponectin | FGF basic | M-CSF |
| Angiopoietin-1 | FGF-19 | MIF |
| Angiopoietin-2 | Fibrinogen | Myeloperoxidase |
| Angiopoietin-like 2 | Growth Hormone | Nidogen-1/Entactin |
| Angiopoietin-like 3 | HGF | Oncostatin M |
| BAFF/BLyS | ICAM-I | Pappalysin-1/PAPP-A |
| BMP-4 | IGFBP-2 | PBEF/Visfatin |
| Cathepsin D | IGFBP-3 | PCSK9 |
| Cathepsin L | IGFBP-4 | Pentraxin-3 |
| Cathepsin S | IGFBP-6 | Pref-1/DLK-1/FA1 |
| CCL2/MCP-1 | IGFBP-rp1/IGFBP-7 | RAGE |
| CCL5/RANTES | IL-1 beta | Resistin |
| Chemerin | IL-6 | Serpin A12 |
| Complement Factor D | IL-10 | Serpin A8/AGT |
| C-Reactive Protein | IL-11 | Serpin E1/PAI-1 |
| CXCL8/IL-8 | LAP (TGF-ß1) | TIMP-1 |
| DPPIV/CD26 | Leptin | TIMP-3 |
| Endocan | LIF | TNF-alpha |
| EN-RAGE | Lipocalin-2/NGAL | VEGF |
| Fetuin B | ||
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
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