| Key Benefits: |
- Qualified for use in organoid cell culture
- Commonly used robust and established organoid systems
- Quality controlled for peformance consistency
Summary for Cultrex Basement Membrane Extract, Type 2, Pathclear
Why Use Cultrex BME, Type 2?
Cultrex Basement Membrane Extract (BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.
Cultrex RGF BME, Type 2 provides a proprietary formulation that has a high storage modulus and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.
Product Specifications for Cultrex Basement Membrane Extract, Type 2, Pathclear
| Species |
Mouse
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| Source |
Murine Engelbreth-Holm-Swarm (EHS) tumor
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| Protein Concentration |
8-12 mg/mL as determined by Lowry assay
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| Endotoxin level |
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
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| Sterility Testing |
No bacterial or fungal growth detected following 14 days in culture
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| Cell Culture Testing |
Organoid Culture - Cultrex BME, Type 2 supports growth and expansion of mouse small intestine organoid progenitor cells.
Gelling Assay - Cultrex BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C.
Dome Assay Cultrex BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates.
Tube Formation Assay - Cultrex BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
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| Viral Testing |
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
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Preparation & Storage
| Stability |
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
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| Shipping Conditions |
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
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| Storage |
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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FAQs for Cultrex Basement Membrane Extract, Type 2, Pathclear
Product Documents for Cultrex Basement Membrane Extract, Type 2, Pathclear
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