Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME), Type R1 is an extracellular matrix hydrogel that is designed for the establishment and expansion of difficult grow organoid cultures. RGF BME Type R1 mimics the in vivo microenvironment and is enriched in entactin to improve take rate and growth of difficult to culture organoid progenitor cells.
Key Benefits:
Qualified for use in organoid cell culture
Improves take-rate and growth in difficult organoid systems
Summary for Cultrex Reduced Growth Factor Basement Membrane Extract, Type R1
Why Use Cultrex RGF BME, Type R1?
Cultrex Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex BME Type R1 provides a proprietary formulation that has a higher storage modulus when compared to our other products Cultrex BME, Cultrex BME Type 2 and Cultrex BME Type 3. Our new Cultrex BME Type R1 has a higher concentration of entactin, one of the BME components that connects laminins and collagens reinforcing the hydrogel structure. Cultrex BME Type R1 has been specifically designed to culture tissue organoids.
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.
Protocols Utilizing Cultrex RGF BME, Type R1 for Organoid Cell Culture.
Organoid-qualified Cultrex RGF BME matrices are ideal scaffolds for organoid and 3D cell culture. R&D Systems offers multiple varieties for organoid culture with Cultrex RGF BME, Type R1 being recommended for more difficult to establish and grow tissue types. Listed below are protocols, designed by our research and development groups, for organoid culture that feature our organoid qualified Cultrex RGF BME matrices.
Product Specifications for Cultrex Reduced Growth Factor Basement Membrane Extract, Type R1
Species
Mouse
Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
8-12 mg/mL as determined by Lowry assay
Endotoxin level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing
Organoid Culture - Cultrex RGF BME, Type R1 supports growth and expansion of mouse small intestine organoid progenitor cells.
Gelling Assay - Cultrex RGF BME, Type R1 gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 7 days at 37°C.
Dome Assay Cultrex RGF BME, Type R1 forms and maintains stable 3-D dome structures on cell culture plates.
Tube Formation Assay - Cultrex RGF BME, Type R1 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Preparation & Storage
Stability
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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