What is the recommended working concentration for Cultrex Laminin I?

The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.

Can Cultrex Mouse Laminin I be used on glass cover slips?

Yes. Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.

Cultrex Stem Cell Qualified Laminin I, Pathclear Best Seller

Name: Cultrex Stem Cell Qualified Laminin I, Pathclear
Brand: R&D Systems
SKU: 3400-010-03

R&D Systems, part of Bio-Techne | Catalog # 3400-010-03

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Product Datasheet / COA / SDS

Mouse Laminin I specifically qualified for use in stem cell applications.

Key Product Details

Features:

Cultrex Stem Cell Qualified Laminin I provides a functionally defined and effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in an undifferentiated state.

Key Benefits:

Qualified to support pluripotent stem cell expansion and differentiation
Pathclear-tested to ensure a pathogen and virus-free material
Source tissue is LDEV-free

Technical Data
Citations / Reviews / FAQs

Summary for Cultrex Stem Cell Qualified Laminin I, Pathclear

Why Use Cultrex Stem Cell Qualified Laminin I?

Cultrex Mouse Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. This highly purified preparation of mouse Laminin I is composed of α1β1γ1 chains with a total molecular weight (MW) of 800 KDa.

Negative for the presence of bacteria and fungi.

Product Specifications for Cultrex Stem Cell Qualified Laminin I, Pathclear

Species	Mouse
Source	Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration	1 mg/mL
Endotoxin level	≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Viral Testing	Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Preparation & Storage

Stability	Product is stable for a minimum of 3 months from date of shipment. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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Cultrex Stem Cell Qualified Laminin I, Pathclear Best Seller

R&D Systems, part of Bio-Techne | Catalog # 3400-010-03

Key Product Details

Summary for Cultrex Stem Cell Qualified Laminin I, Pathclear

Why Use Cultrex Stem Cell Qualified Laminin I?

Product Specifications for Cultrex Stem Cell Qualified Laminin I, Pathclear

Preparation & Storage

Customer Reviews for Cultrex Stem Cell Qualified Laminin I, Pathclear

FAQs for Cultrex Stem Cell Qualified Laminin I, Pathclear

Product Documents for Cultrex Stem Cell Qualified Laminin I, Pathclear

Product Datasheets

COA

Certificate of Analysis

SDS

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