DMEM/F-12, 15 mM HEPES, GlutaminePlus, no phenol red

Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F-12 (DMEM/F-12) is a 1:1 mixture of DMEM and F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham’s Nutrient Mixture F-12’s additional components, was found to support the growth of a wide range of mammalian cells. DMEM/F-12 requires supplementation with serum, typically 10% Fetal Bovine Serum (FBS), since this medium does not contain proteins, lipids, or growth factors.

Each lot of DMEM/F-12 is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.2 micron filter. Representative samples of each lot of DMEM/F-12 are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. DMEM/F-12 is manufactured in our ISO 9001:2015 certified facility.

For the specific media formulation, please refer to the datasheet.