| Key Benefits: |
- Ready-to-use: Complete all-in-one formulation, no growth factor supplementation required
- Animal-free GMP: Ideal for pre-clinical to clinical research
- Robust: Superior expansion and maintenance of undifferentiated iPSCs
- Consistent: Reproducible results
- Flexible: Compatible with a variety of matrices and cell lines
- Stable: Maintains cell viability, genotype and differentiation potential over long-term culture
Summary for ExCellerate™ iPSC Expansion Medium, Animal-Free, GMP
Why Use ExCellerate™ iPSC Expansion Medium?
ExCellerate™ iPSC Expansion Medium, Animal-Free, GMP is specifically formulated for the in vitro, feeder-free expansion of human pluripotent stem cells, including embryonic stem (ES) and induced pluripotent stem (iPS) cells. The formulation is complete and contains the growth factors required to support iPSC expansion and maintenance in a single, ready-to-use format. This simplifies stem cell culture workflows and provides a reproducible cell culture environment to ensure consistent performance.
No materials of animal or human origin are used in the production of this medium or its components. This medium is produced in a dedicated Animal-Free production space. Elimination of animal-derived components reduces variability and improves lot-to-lot consistency for reliable generation of high quality, homogenous, and undifferentiated cells needed for any stage of research. Animal-free traceability of raw materials reduces risk and makes it ideal for translational research. An RUO version is also available to support a seamless transition into clinical applications.
The composition of ExCellerate™ iPSC Expansion Medium is optimized to support the robust expansion of pluripotent stem cells, while maintaining cell viability, genotype and differentiation potential for extended times in vitro. WiCell has concluded that ExCellerate™ iPSC Expansion Medium has met all WiCell requirements for quality and is appropriate for pluripotent stem cell culture.
This medium is compatible with various cell culture matrices, including Cultrex Ultimatrix, Cultrex ReadyBME and Recombinant Human Vitronectin, and cell lines, making it versatile for use in research, preclinical, and translational stem cell culture workflows.
Kit Contents for ExCellerate™ iPSC Expansion Medium, Animal-Free, GMP
500 mL of ExCellerate iPSC Expansion Media
Product Specifications for ExCellerate™ iPSC Expansion Medium, Animal-Free, GMP
| Species |
Human
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| Osmolality |
280-350
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Limitations
For preclinical, or clinical ex vivo use. Not for in vivo use. - The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
- This reagent should not be used beyond the expiration date indicated on the CoA.
- Results may vary due to variations among pluripotent stem cell populations derived from different donors.
Scientific Data Examples for ExCellerate™ iPSC Expansion Medium, Animal-Free, GMP
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Human iPSC expanded in CCM036 and CCM036-GMP show similar growth kinetics.
Catalog # CCM036 (RUO version) and Catalog # CCM036-GMP have the same formulation and are manufactured with the same high quality animal-free components. No significant difference in growth kinetics is seen when culturing iPSC in either CCM036 or CCM036-GMP.
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Using ExCellerate iPSC Media better supports low density iPSC culture compared to other media suppliers.
Human iPSCs were plated at low density (3000 cells per well in a 6 well plate) and cultured until the cells reached approximately 70% confluence for 3 passages. Cells were harvested using Accutase and live cells were counted. All groups were then normalized to CCM036-GMP. Competitors showed slower and highly variable cell growth (Plot A) as well as lower viability (Plot B) compared to CCM036-GMP.
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ExCellerate™ iPSC Expansion Medium maintain expression of stemness markers over long-term cultures (at least 24-passages).
Human iPSC expanded in CCM036-GMP express key stemness markers, Oct 3/4 and Nanog. SSEA-1 used as negative control.
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Stable genotype over long-term culture.
Human iPSCs cultured in CCM036-GMP show characteristics healthy colony morphology including clearly defined edges, compact and tightly packed cells, along with normal karyotype after 26 passages.
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Representative images of iPSC expansion and differentiation.
Image A shows iPSCs expanded in culture using ExCellerate iPSC Expansion Medium (Catalog # CCM036-GMP).
Image B shows the differentiation into PDX-1 expressing pancreatic progenitors (AF2419).
Image C shows the presence of large population of C-peptide (MAB14171) expressing monohormonal beta cells.
Image D shows the co-expression of PDX-1 and C-peptide in the population.
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iPSC grown in CCM036-GMP retain their pluripotency.
After expansion in CCM036-GMP, cells were differentiated to the three germ layers: endoderm (SOX17), mesoderm (Brachyury), and ectoderm (OTX2).
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Preparation & Storage
| Shipping Conditions |
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
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| Storage |
Store the unopened product at ≤ -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store opened product up to 2 weeks at 2-8 °C.
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Product Documents for ExCellerate™ iPSC Expansion Medium, Animal-Free, GMP
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