ITS-Plus Media Supplement (100X)
R&D Systems, part of Bio-Techne | Catalog # AR014
Key Product Details
Summary for ITS-Plus Media Supplement (100X)
Supports cell proliferation under low serum conditions.
Key Benefits
- Includes linoleic acid and BSA for enhanced cell health in low serum conditions
- Contains optimized concentrations of insulin, transferrin, and selenite
- Chemically-defined cell culture media decreases experimental variation
- High lot-to-lot consistency
- Performance tested on multiple cell lines
Why Use Pre-optimized and Serum-free Supplements for Cell Culture?
The efficiency of cell proliferation and maintenance of cell health can vary from one experiment to another depending on the media components, the supplements used to drive proliferation, and the quality of the starting cell population.
Although commonly used, serum-containing medium is inherently variable due to the undefined composition and lot-to-lot variability across batches of serum. Troubleshooting media variability can be a costly and time-consuming obstacle that can be minimized through the use of high quality media. Our ITS-Plus Media Supplement is a pre-optimized and serum-free cell culture media supplement that was designed to help maintain media consistency across passages and experiments. The ITS-Plus Media Supplement contains optimized concentrations of human insulin, human transferrin, selenite, linoleic acid, and BSA.
ITS-Plus Media Supplement (100x):
- Includes linoleic acid and BSA for enhanced cell health in low serum conditions
- Contains optimized concentrations of insulin, transferrin, and selenite
- Chemically-defined cell culture media decreases experimental variation
- High lot-to-lot consistency
- Performance tested on multiple cell lines
5 mL ITS-Plus Media Supplement (100x)
| Component | Amount (mg/mL) |
| Recombinant Human Insulin | 1.0 |
| Human Transferrin | 0.5 |
| Selenite | 6.7 x 10-4 |
| Linoleic Acid | 0.5 |
| BSA | 100 |
Stability and Storage
Store in the dark at °C in a manual defrost freezer. Do not use past the expiration date.
Precautions
This product contains human transferrin. This transferrin was purified from donor plasma and tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen.
Limitations
- FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
- The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
- This reagent should not be used beyond the expiration date indicated on the label.
|
ITS-Plus Media Supplement Enhances Cell Viability Under Low Serum and Stress-induced Conditions. ITS-Plus Media Supplement Enhances Cell Viability Under Low Serum and Stress-induced Conditions.MDA-MB-231 cells (1 x 105/mL) were cultured for 3 days in serum-free medium, media with ITS-Plus Media Supplement (Catalog # AR014), or media with standard ITS Supplement. For each media condition, viable cells were counted in cultures with media alone (blue bars) or in media plus indomethacin (25 µg/mL; red bars). Higher cell counts were obtained in cultures supplemented with ITS-Plus Media Supplement in both media alone and in cultures with indomethacin added. |
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, ITS Media Supplement is prepared using the following procedure:
- Dilute the media supplement in basal media
- Store completed media at 2 – 8 °C
Reagents provided in the ITS-Plus Media Supplement (Catalog # AR014):
| Component | Amount (mg/mL) |
| Recombinant Human Insulin | 1.0 |
| Human Transferrin | 0.5 |
| Selenite | 6.4 x 10-4 |
| Linoleic Acid | 0.5 |
| BSA | 100 |
Stability and Storage
Store in the dark at < -20 °C in a manual defrost freezer. Do not use past the expiration date.
Precautions
This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious reagents, these reagents should be handled as if capable of transmitting infection.
Materials
- Basal media
- Serological pipettes
- Pipettes and pipette tips
Equipment
- 2 °C to 8 °C refrigerator
Dilute 100-fold into cell culture media of interest before use. The final concentration of serum required with ITS supplementation is cell type and assay dependent. Titrate for individual use.
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