N21-MAX Insulin Free Media Supplement (50X)
R&D Systems, part of Bio-Techne | Catalog # AR010
Key Product Details
Summary for N21-MAX Insulin Free Media Supplement (50X)
For the reliable maturation and superior long-term maintenance of primary neurons under insulin free culture conditions.
Key Benefits
- Ideal for studies of insulin secretion or insulin receptor function
- Optimized formulation of the original B27 recipe
- Rigorous quality control testing ensures consistency across experiments
- Eliminates the need for an astrocyte feeder layer
- Is nutrient-rich, serum-free, and fully defined
Why Culture Neurons in Serum-Free N21-MAX Media Supplement?
The N21-MAX Insulin Free Media Supplement improves upon the traditional B271 and NS212 neuronal supplements, offering a serum-free, insulin-free, and fully-defined formulation that is optimized for the reliable maturation, consistent health, and superior function of neurons in culture. This insulin-free formulation is ideal insulin-sensitive research, including studies focused on insulin secretion or insulin receptor function.
N21-MAX was designed to eliminate uncontrolled variables, such as those found in both serum-containing media and in some commercial serum-free neuronal supplements. Use of undefined media supplements can confound neuronal growth and maturation. N21-MAX contains a cocktail of factors optimized for neuronal survival, neurite extension, and synaptic maturation. Each lot of N21-MAX is tested on primary hippocampal neurons to ensure consistent performance for the user.
The N21-MAX Insulin Free Media Supplement:
- Is fully defined and quality tested to reduce unwanted experimental variation.
- Contains factors qualified to support reproducible and long-term neuron culture.
- Modified from the published B27 formulation1.
- Does not require an astrocyte feeder layer.
- Has been tested to support the viability and growth of E18 rat hippocampal neurons.
- Brewer et al., (1993) J. Neurosci. Res. 32:567
- Chen et al., (2008) J. Neurosci. Methods 171:239
N21-MAX Insulin Free Media Supplement Components
N21-MAX Insulin Free Media Supplement is optimized for the maturation and long-term culturing of neuronal cells. The supplement is supplied as a 50X concentrated solution and contains the following 20 components:
- Albumin (bovine)
- L-Carnitine
- Catalase
- Corticosterone
- Ethanolamine
- Glutathione
- Galactose
- Holo-Transferrin
- Linoleic Acid
- Lipoic Acid
- Progesterone
- Putrescine
- Retinyl acetate
- Retinol
- Selenite
- Superoxide dismutase
- Triiodo-L-thyronine
- D,L-alpha-Tocopherol
- D,L-alpha-Tocopherol acetate
Supplied in a volume sufficient to supplement 500 mL of media at the recommended concentration. N21-MAX Insulin Free Media Supplement is tested for use in Neurobasal Media.
Precautions
This product contains human transferrin. This transferrin was purified from donor plasma and tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen.
Neural stem cells provide an excellent model for research focused on neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. In addition, primary mouse cortical stem cells isolated from E14.5 CD-1 mice are available. Every lot of R&D Systems Cortical Stem Cells is validated for a high level of Nestin expression and the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. Our cortical stem cells are tested to ensure highest quality and lot to lot consistency. Both rat and mouse Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.
To complement the use of primary neural stem cells, we offer a range of supportive products, including culture media which is specifically optimized for use with neural stem cells. We offer kits to promote the in vitro proliferation of neural precursors, and kits to differentiate neural stem cells into dopaminergic neurons or oligodendrocytes. In addition, kits are available which contain panels of antibodies designed to monitor the differentiation and identification of neural precursors, astrocytes, neurons, and oligodendrocytes.
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Synaptotagmin Expression in Neurons Cultured in Media Supplemented with N21-MAX Insulin Free Media Supplement. Synaptotagmin Expression in Neurons Cultured in Media Supplemented with N21-MAX Insulin Free Media Supplement.E18 rat hippocampal neurons were grown for 21 days in vitro in media supplemented with N21-MAX Insulin Free Media Supplement. The cells were stained with the Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (R&D Systems, Catalog # MAB4364), followed by the NorthernLights™ (NL)557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; R&D Systems, Catalog # NL007). The nuclei were counterstained with DAPI. |
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, completed N21-MAX Insulin Free-supplemented medium is prepared using the following procedure:
- Dilute the media supplement in basal media
- Store completed media
- Use within 2 weeks in the dark
Reagents provided in the N21-MAX Insulin Free Media Supplement (Catalog # AR010):
- Albumin (bovine)
- L-Carnitine
- Catalase
- Corticosterone
- Ethanolamine
- Glutathione
- Galactose
- Holo-Transferrin
- Linoleic Acid
- Linolenic Acid
- Lipoic Acid
- Progesterone
- Putrescine
- Retinyl acetate
- Retinol
- Selenite
- Superoxide dismutase
- Triiodo-L-thyronine
- D,L-alpha-Tocopherol
- D,L-alpha-Tocopherol acetate
Reagents
- Basal media (e.g., Neurobasal Media from Invitrogen®, Catalog # 21103-029 or equivalent)
Materials
- Serological pipettes
- Pipettes and pipette tips
Equipment
- 2 °C to 8 °C refrigerator
Dilute 50-fold with a basal media (e.g., Neurobasal Media) and supplement with 0.5 mM L-glutamine before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.
Invitrogen is a registered trademark of Invitrogen Corp.