Sodium Bicarbonate, powder

Sodium bicarbonate improves the pH control of media incubated in a 5-10% CO₂ atmosphere and provides cells with carbonate ions that are essential for the metabolic functions of most cells. Additionally, this buffer is non-toxic to cells in culture. Since most pre-mixed powdered media are manufactured without sodium bicarbonate, this buffer must be added in appropriate concentrations during liquid media preparation either as a sterile solution or as a powder prior to filtration.