TcBuster™-M GMP Transposase mRNA

Gene delivery is a crucial step for a wide variety of genome engineering workflows, which have traditionally utilized viral vectors to achieve expression of exogenous DNA sequences. However, the generation of a virus is time consuming, inconsistent, and has limited cargo capacity. TcBuster-M Transposase mRNA, in combination with a compatible DNA TcBuster transposon (plasmid), form the TcBuster non-viral gene delivery system. The TcBuster non-viral gene delivery system is a solution for stable integration of DNA cargo and addresses many of the limitations of viral vectors. This product requires a TcBuster DNA transposon to be functional. Research-grade mRNA and DNA transposon is available for testing from Bio-Techne. For an equivalent RUO version, please view TcBuster-M Transposase mRNA. For control transposons, check out the CD19CAR-DHFR-eGFP Control for cell therapy applications or Insert On eGFP Control for immortalized cell applications. Please contact us for more information on custom or GMP transposons at techsupport@bio-techne.com.

Gene delivery is a critical step in engineering cells for a wide variety of applications. For T and NK immunotherapies, delivery of CAR or TCR DNA cargo is crucial for the potency of the end drug product. Genome engineering involving iPSCs can require stable delivery of complex DNA cargo. For biologics, this system can faciliate stable gene transfer and high titers for work in CHO and HEK cell lines. Because of the diverse applications of the TcBuster system, independent experimental optimization and/or process development will be required. Please visit the TcBuster Non-Viral Gene Engineering page for more information on the system.

For preclinical or clinical ex vivo use. Not for in vivo use.

• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• Refer to the Certificate of Analysis for the Use by Date indicated.
• Results may vary due to variations among pluripotent stem cell populations derived from different donors.