Mouse Aquaporin-0 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69841

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

View all Aquaporin-0 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse AQP-0 in samples. No significant cross-reactivity or interference between Mouse AQP-0 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.62%

Inter-Assay Precision (Precision between assays) CV% < 4.56%

Recovery for Mouse Aquaporin-0 ELISA Kit (Colorimetric)

Recovery

83-102%

Linearity

Mouse Aquaporin-0 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Aquaporin-0 ELISA Kit (Colorimetric)

Mouse Aquaporin-0 ELISA Kit (Colorimetric)

ELISA: Mouse Aquaporin-0 ELISA Kit (Colorimetric) [NBP2-69841] -

ELISA: Mouse Aquaporin-0 ELISA Kit (Colorimetric) [NBP2-69841] - Standard Curve Reference

Kit Contents for Mouse Aquaporin-0 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Aquaporin-0

Aquaporins (AQPs) are a large family of integral membrane channel proteins that facilitate the transport of water through the cell membrane. Aquaporins are widely distributed and are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-Calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.

Alternate Names

aquaporin 0, lens fiber major intrinsic protein, major intrinsic protein of lens fiber, MP26

Gene Symbol

MIP

Additional Aquaporin-0 Products

Product Documents for Mouse Aquaporin-0 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse Aquaporin-0 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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