Human HGF Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DHG00
Key Product Details
Assay Length
4.25 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Citrate Plasma (50 µL)
Sensitivity
40 pg/mL
Assay Range
125-8000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Product Summary for Human HGF Quantikine ELISA Kit
The Quantikine Human HGF Immunoassay is a 4.25 or 4.5 hour solid phase ELISA that is designed to measure HGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human pro-HGF and antibodies raised against the recombinant factor and has been shown to quantitate recombinant human HGF accurately. Results obtained using natural HGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human HGF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human HGF in this assay. No medical histories were available for the donors used in this
study.
Cell Culture Supernates - Human peripheral blood mononuclear cells (5 x 106 cells/mL) were cultured in RPMI supplemented with 5% fetal calf serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human HGF.
ND=Non-detectable
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=60) | 1257 | 671-1992 | 289 |
| EDTA plasma (n=60) | 787 | 469-113 | 136 |
| Citrate plasma (n=71) | 440 | 251-742 | 102 |
Cell Culture Supernates - Human peripheral blood mononuclear cells (5 x 106 cells/mL) were cultured in RPMI supplemented with 5% fetal calf serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human HGF.
| Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | ND | 548 |
| Stimulated | 152 | 145 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 425 | 991 | 3869 | 409 | 1058 | 4058 |
| Standard Deviation | 30.2 | 43.2 | 234 | 29.4 | 75.1 | 264 |
| CV% | 7.1 | 4.4 | 6.0 | 7.2 | 7.1 | 6.5 |
Citrate Plasma, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 339 | 999 | 3759 | 363 | 967 | 3790 |
| Standard Deviation | 23.7 | 56.2 | 154 | 25.8 | 81.2 | 205 |
| CV% | 7.0 | 5.6 | 4.1 | 7.1 | 8.4 | 5.4 |
Recovery for Human HGF Quantikine ELISA Kit
The recovery of HGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 92 | 86-101 |
| Citrate Plasma (n=5) | 105 | 99-114 |
| EDTA Plasma (n=5) | 100 | 85-111 |
| Serum (n=5) | 99 | 91-108 |
Linearity
To assess the linearity of the assay, five samples of each sample type were spiked with high concentrations of HGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human HGF Quantikine ELISA Kit
Human HGF ELISA Standard Curve
Human HGF ELISA Cell Culture Supernate Standard Curve
Human HGF ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HGF
Long Name
Hepatocyte Growth Factor
Alternate Names
DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF
Entrez Gene IDs
Gene Symbol
HGF
Additional HGF Products
Product Documents for Human HGF Quantikine ELISA Kit
Product Specific Notices for Human HGF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.