Human IFN-alpha All Subtype Quantikine HS ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # HSFNAS0
Key Product Details
Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
0.17 pg/mL
Assay Range
0.938-60 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IFN-alpha All Subtype Quantikine HS ELISA Kit
The Quantikine™ HS Human IFN-alpha All Subtype Immunoassay is a 4.0 hour solid phase ELISA designed to measure all human IFN-alpha subtypes in serum and plasma. It contains HEK293-expressed recombinant human IFN-alpha 2a and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-alpha subtypes.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Reactivity
Human
Specificity
Natural and recombinant human IFN-alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 7.68 | 14.7 | 29.1 | 8.10 | 15.8 | 31.3 |
| Standard Deviation | 0.175 | 0.377 | 0.665 | 0.357 | 0.728 | 1.70 |
| CV% | 2.3 | 2.6 | 2.3 | 4.4 | 4.6 | 5.4 |
Recovery for Human IFN-alpha All Subtype Quantikine HS ELISA Kit
The recovery of human IFN-alpha spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 92 | 84-97 |
| Heparin Plasma (n=4) | 90 | 81-99 |
| Serum (n=4) | 93 | 85-104 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human IFN-alpha were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IFN-alpha All Subtype Quantikine HS ELISA Kit
Human IFN-alpha All SubType ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-alpha
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Human IFN-alpha All Subtype Quantikine HS ELISA Kit
Product Specific Notices for Human IFN-alpha All Subtype Quantikine HS ELISA Kit
For research use only
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