Human IL-6 Quantikine HS ELISA
R&D Systems, part of Bio-Techne | Catalog # HS600B
Key Product Details
Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Serum (100 µL), EDTA Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)
Sensitivity
0.11 pg/mL
Assay Range
0.156-10 pg/mL (Serum, EDTA Plasma, Citrate Plasma, Urine)
Product Summary for Human IL-6 Quantikine HS ELISA
The Quantikine HS Human IL-6 Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human IL-6 in serum, plasma, and urine. It contains E. coli-expressed recombinant human IL-6 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-6 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IL-6.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-6
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Citrate Plasma, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 36 | 36 | 36 |
| Mean (pg/mL) | 0.436 | 2.45 | 5.53 | 0.490 | 2.78 | 5.65 |
| Standard Deviation | 0.030 | 0.19 | 0.41 | 0.047 | 0.20 | 0.37 |
| CV% | 6.9 | 7.8 | 7.4 | 9.6 | 7.2 | 6.5 |
Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 35 | 35 | 35 |
| Mean (pg/mL) | 0.526 | 3.09 | 5.77 | 0.502 | 2.78 | 5.62 |
| Standard Deviation | 0.052 | 0.18 | 0.32 | 0.056 | 0.19 | 0.31 |
| CV% | 9.8 | 5.8 | 5.5 | 11.2 | 6.8 | 5.5 |
Recovery for Human IL-6 Quantikine HS ELISA
The recovery of IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Citrate Plasma (n=8) | 106 | 90-125 |
| EDTA Plasma (n=8) | 97 | 84-113 |
| Serum (n=8) | 94 | 87-99 |
| Urine (n=8) | 102 | 93-112 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-6 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-6 Quantikine HS ELISA
Human IL-6 High Sensitivity ELISA Assay
Human IL-6 ELISA Serum/Plasma Assay
Human IL-6 ELISA Urine Assay
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
R&D Systems, a Bio-Techne brand, offers a comprehensive range of IL-6 Antibody products, IL-6 Proteins and Enzymes, IL-6 ELISAs, IL-6 Luminex Assays and more. Use the filter on the left to drill down to the IL-6 product that best fits your needs.
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Entrez Gene IDs
Gene Symbol
IL6
Additional IL-6 Products
Product Documents for Human IL-6 Quantikine HS ELISA
Product Specific Notices for Human IL-6 Quantikine HS ELISA
For research use only
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