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Human Lactalbumin Alpha ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18210

Novus Biologicals, part of Bio-Techne
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NBP3-18210

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and cell culture supernates (100 ul)

Sensitivity

0.38 ng/ml

Assay Range

0.78-50 ng/ml

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Lactalbumin Alpha in the sample, this Human Lactalbumin Alpha ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Lactalbumin Alpha concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 15

Recovery for Human Lactalbumin Alpha ELISA Kit (Colorimetric)

Recovery

80-109%

Scientific Data Images for Human Lactalbumin Alpha ELISA Kit (Colorimetric)

ELISA: Human Lactalbumin Alpha ELISA Kit (Colorimetric) [NBP3-18210]

ELISA: Human Lactalbumin Alpha ELISA Kit (Colorimetric) [NBP3-18210]

ELISA: Human Lactalbumin Alpha ELISA Kit (Colorimetric) [NBP3-18210] - Standard Curve Reference

Kit Contents for Human Lactalbumin Alpha ELISA Kit (Colorimetric)

  • Balance Solution
  • Enzyme Conjugate
  • Microtiter Plate
  • Standards
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Lactalbumin Alpha

Alpha-LA is the B protein of lactose synthetase secreted by the mammary epithelial cells. a-LA is a potent Ca(2+)-elevating and apoptosis-inducing agent with broad, yet selective, cytotoxic activity. Multimeric a-LA kills all transformed, embryonic, and lymphoid cells tested but spared mature epithelial elements. It suggests that milk contributes to mucosal immunity not only by furnishing antimicrobial molecules but also by policing the function of lymphocytes and epithelium. a-LA may be helpful in ascertaining the site of origin of metastatic breast tumors

Alternate Names

alpha-lactalbumin, lactalbumin, alpha-, Lactose synthase B protein, Lysozyme-like protein 7, LYZL7, MGC138521, MGC138523

Gene Symbol

LALBA

Additional Lactalbumin Alpha Products

Product Documents for Human Lactalbumin Alpha ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Lactalbumin Alpha ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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