Mouse M-CSF Quantikine ELISA Kit

Name: Mouse M-CSF Quantikine ELISA Kit
Brand: R&D Systems
SKU: MMC00
Rating: 5 (3 reviews)

R&D Systems, part of Bio-Techne | Catalog # MMC00

(3)

Citations (34)

Product Datasheet / COA / SDS

Discontinued Product

MMC00 has been discontinued. An alternative/replacement product is available: MMC00B. View all M-CSF products.

Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (13 µL), Serum (13 µL), EDTA Plasma (13 µL)

Sensitivity

5 pg/mL

Assay Range

31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

View all M-CSF ELISA Kits »

Product Summary for Mouse M-CSF Quantikine ELISA Kit

The Quantikine Mouse M-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse M-CSF levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse M-CSF and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse M-CSF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse M-CSF.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Mouse

Specificity

Natural and recombinant mouse M-CSF

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	44	174	558	46	174	554
Standard Deviation	3.9	10.2	38.9	4.0	10.1	35.2
CV%	8.9	5.9	7.0	8.7	5.8	6.4

Recovery for Mouse M-CSF Quantikine ELISA Kit

The recovery of mouse M-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	104	85-120
EDTA Plasma (n=6)	101	89-112
Serum (n=6)	97	85-115

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse M-CSF in each matrix were diluted with Calibrator Diluent and then assayed.

Scientific Data Images for Mouse M-CSF Quantikine ELISA Kit

Mouse M-CSF ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Gene Symbol

CSF1

Additional M-CSF Products

Product Documents for Mouse M-CSF Quantikine ELISA Kit

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse M-CSF Quantikine ELISA Kit

For research use only

Citations
Reviews

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.