Human Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81237

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all Pyruvate Dehydrogenase Phosphatase/PDP1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C in samples. No significant cross-reactivity or interference between Human Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.77%

Inter-Assay Precision (Precision between assays) CV% < 4.8%

Recovery

Recovery

87-104%

Linearity

Human Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (Colorimetric)

Scientific Data Images

Human Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (Colorimetric)

ELISA: Human Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (Colorimetric) [NBP2-81237] -

ELISA: Human Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C ELISA Kit (Colorimetric) [NBP2-81237] - Standard Curve Reference

Kit Contents

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Pyruvate Dehydrogenase Phosphatase/PDP1

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.(provided by RefSeq)

Alternate Names

PDH, PDP1, PDPC 1, PPM2C

Gene Symbol

PDP1

Additional Pyruvate Dehydrogenase Phosphatase/PDP1 Products

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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