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Fixation and Permeabilization Flow Cytometry Kit

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31377

Novus Biologicals, part of Bio-Techne
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NBP2-31377

Key Product Details

Applications

Flow Cytometry

Kit Type

Flow Cytometry Kit

Product Summary for Fixation and Permeabilization Flow Cytometry Kit

Biological fluids (blood, bone marrow, and others) must be collected under sterile conditions. Anticoagulation with EDTA or heparin is recommended. The samples should be stored at room temperature until used. For optimal results, samples should be processed and analyzed within 24 hours. Samples with high numbers of non-viable cells might cause false results, such cases require determination of cell viability with e.g. propidium iodide. All biological samples have to be handled with caution. Always consider them as potentially infective. Use appropriate precautions such as gloves, lab-coat, etc.

Product Specifications

Application Notes

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Scientific Data Images for Fixation and Permeabilization Flow Cytometry Kit

Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377]

Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377]

Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Double labeling of a normal blood sample treated with NBP2-31377, and immunostained for Lactoferrin (PE) and Lysozyme (FITC).
Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377]

Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377]

Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Flow cytrometric analysis of normal bone marrow (BM) after fixation and pemeabilization using NBP2-31377, followed by immunostaining for lactoferrin and MPO-C2.
Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377]

Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377]

Flow Cytometry: Fixation and Permeabilization Flow Cytometry Kit [NBP2-31377] - Flow cytrometric analysis of bone marrow (BM) from a leukemia patient after fixation and pemeabilization using NBP2-31377, followed by immunostaining for lactoferrin and MPO-C2

Kit Contents for Fixation and Permeabilization Flow Cytometry Kit

  • Reagent A: Fixation Medium (1 x 5ml vial)
  • Reagent B: Permeabilization Medium (1 x 5ml vial)

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at room temperature.

Background: Fixation and Permeabilization

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the Fixation and Permeabilization Flow Cytometry Kit, flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the Fixation and Permeabilization Flow Cytometry Kit; some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

Alternate Names

FIX&PERM

Additional Fixation and Permeabilization Products

Product Documents for Fixation and Permeabilization Flow Cytometry Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fixation and Permeabilization Flow Cytometry Kit

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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