Intracellular NK Cell Activation Flow Cytometry Panel
R&D Systems, part of Bio-Techne | Catalog # FMC-P-005
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Intracellular NK Cell analysis over a 14-day time course. NK cells were expanded from PBMCs using plate-bound Anti-Human NKp46 Monoclonal Antibody (MAB1850) in ExCellerate™ Human NK Cell Expansion Media, Xeno-Free (CCM032) plus Recombinant Human IL-2 (27 ng/mL, 202-IL), Recombinant Human IL-12 (10 ng/mL, 219-IL), Recombinant Human IL-18 (10 ng/mL, 9124-IL), Recombinant Human IL-21 (10 ng/mL, 8879-IL) for 13 days. NK cells were assessed on Days 0 and 13 for expression of human CD16 PerCP, Granzyme K Alexa Fluor® 594, Granzyme B Alexa Fluor 488, Perforin Alexa Fluor 750, CD56 Alexa Fluor 647, and CD3 Alexa Fluor 405. Cells were gated on singlets (FSC-A by FSC-H) and live cells and quadrants were set using isotype controls (not shown). Find Staining Protocol Here. |
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