5-TAMRA NHS

Name: 5-TAMRA NHS
Brand: Tocris Bioscience
SKU: 7665

Tocris Bioscience, part of Bio-Techne | Catalog # 7665

COA / SDS

Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides, FRET acceptor for FAM

Discontinued Product

7665 has been discontinued. View all Fluorescent Probes products.

Key Product Details

Description:	Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides, FRET acceptor for FAM
Chemical Name:	2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate
Purity:	≥90% (HPLC)
Molecular Weight:	527.52

View Full Technical Details for 5-TAMRA NHS

Technical Data
Citations

Biological Activity for 5-TAMRA NHS

Key information: 5-TAMRA NHS is an orange fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines. Can be used for oligonucleotide labeling.

Application: Suitable for fluorescence microscopy, confocal microscopy, FRET acceptor for FAM.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 546 nm and 580 nm, respectively; quantum yield = 0.1; extinction coefficient = 95,000 M^-1cm^-1; A280 = 0.178.

Please see the protocol for further information on protein/antibody labeling and conjugation.

It is recommended to prepare stock solutions in DMSO.

Optical Data for 5-TAMRA NHS

Emission Color	Orange
Brightness
λ_abs	546 nm
λ_em	580 nm
Extinction Coefficient (ε)	95,000 M^-1cm^-1
Quantum Yield (φ)	0.1
Closest Laser line	532 nm
Reactive Group	NHS ester
Reactivity	Primary amines
Cell Permeable	Yes
pH Sensitivity	No
Correction Factor 280	0.178
Application	Fluorescence Microscopy, Confocal Microscopy
Alternative For	Alexa Fluor® 546

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for 5-TAMRA NHS

M. Wt	527.52
Formula	C₂₉H₂₅N₃O₇
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	150810-68-7
PubChem ID	2762600
InChi Key	VWFRSNKRTNUMET-UHFFFAOYSA-N
Smiles	CN(C)C1=CC=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C([O-])=O)=C(C=C/4)C(O5)=CC4=[N+](C)\C)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for 5-TAMRA NHS

References are publications that support the biological activity of the product.

Hirata Structural evaluation of tandem hairpin pyrrole-imidazole polyamides recognizing human telomeres. J.Am.Chem.Soc. 2014 PMID: 25036716
Kvach Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug.Chem. 2009 PMID: 19606815