ALDH Detection Reagent - BAAA-DA

Name: ALDH Detection Reagent - BAAA-DA
Brand: Tocris Bioscience
SKU: 7556

Tocris Bioscience, part of Bio-Techne | Catalog # 7556

COA / SDS

Fluorescent ALDH substrate and detection reagent. Used for: identification of cells with high ALDH activity. Application: flow cytometry, fluorescent-activated cell sorting (FACS)

Key Product Details

Description:	Fluorescent ALDH substrate and detection reagent. Used for: identification of cells with high ALDH activity. Application: flow cytometry, fluorescent-activated cell sorting (FACS)
Chemical Name:	(T-4)-[N-(2,2-Diethoxyethyl)-5-[(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrole-2-propanamidato-κN¹]difluoroboron
Purity:	≥95% (HPLC)
Molecular Weight:	407.27

View Full Technical Details for ALDH Detection Reagent - BAAA-DA

Technical Data
Citations

Biological Activity for ALDH Detection Reagent - BAAA-DA

Key information: ALDH Detection Reagent - BAAA-DA is a fluorescent substrate of aldehyde dehydrogenase (ALDH).

Used for: can be used in flow cytometry to sort cancer stem cells with high ALDH levels, and to isolate human hematopoietic progenitor cells. Can be used with efflux inhibitors such as Verapamil (Cat. No. 0654), Probenecid (Cat. No. 4107) or 2-deoxy-D-glucose (Cat. No. 4515) to block multi-drug resistance efflux.

Application: flow cytometry, fluorescent-activated cell sorting (FACS).

Properties and Photophysical Data: BAAA-DA is converted under acidic conditions to BAAA. BAAA, cell-permeant, is converted by intracellular ALDH into carboxylate ion amino acetate (BAA) and used to identify cells with high ALDH activity. Excitation and emission maxima (λ) are 505 nm and 512 nm, respectively; extinction coefficient = 74,595 M^-1cm^-1.

Optical Data for ALDH Detection Reagent - BAAA-DA

λ_abs	505 nm
λ_em	512 nm
Extinction Coefficient (ε)	74595 M^-1cm^-1

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Technical Data for ALDH Detection Reagent - BAAA-DA

M. Wt	407.27
Formula	C₂₀H₂₈BF₂N₃O₃
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	247069-93-8
PubChem ID	91759536
InChi Key	LHPURSCMUYRRRM-UHFFFAOYSA-N
Smiles	CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(NCC(OCC)OCC)=O)C=CC3=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ALDH Detection Reagent - BAAA-DA

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	4.07	10

Preparing Stock Solutions for ALDH Detection Reagent - BAAA-DA

The following data is based on the product molecular weight 407.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	24.55 mL	122.77 mL	245.54 mL
0.5 mM	4.91 mL	24.55 mL	49.11 mL
1 mM	2.46 mL	12.28 mL	24.55 mL
5 mM	0.49 mL	2.46 mL	4.91 mL

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References for ALDH Detection Reagent - BAAA-DA

References are publications that support the biological activity of the product.

Storms Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity. Proc.Natl.Acad.Sci.U.S.A. 1999 PMID: 10430905