Calcein AM
Tocris Bioscience, part of Bio-Techne | Catalog # 5119
Key Product Details
| Description: | Cell permeable non-fluorescent compound; green-fluorescent in living cells once hydrolyzed. Used for: cell tracing and cell viability monitoring. Application: fluorescent microscopy and flow cytometry |
| Chemical Name: | N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 994.86 |
Biological Activity for Calcein AM
Key information: Calcein AM is a cell permeable non-fluorescent compound, that becomes green fluorescent once hydrolyzed in live cells.
Used for: cell tracing, monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance.
Application: fluorescent microscopy and flow cytometry.
Properties and Photophysical Data: in live cells, non-fluorescent Calcein AM is hydrolyzed by intracellular esterases into green-fluorescent calcein, which is retained in the cytoplasm. Excitation and emission maxima (λ) are 495 nm and 515 nm, respectively.
It is recommended to prepare stock solutions of Calcein AM in DMSO.
Optical Data for Calcein AM
| Emission Color | Green |
|---|---|
| λabs | 495 nm |
| λem | 515 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Fluorescent Microscopy |
Plan Your Experiments
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