Cyanine 5 Tyramide

Name: Cyanine 5 Tyramide
Brand: Tocris Bioscience
SKU: 6458

Tocris Bioscience, part of Bio-Techne | Catalog # 6458

Citations (2)

COA / SDS

Red fluorescent reagent widely used for signal amplification in IHC and FISH

Key Product Details

Description:	Red fluorescent reagent widely used for signal amplification in IHC and FISH
Alternative Names:	Cy5 Tyramide
Chemical Name:	2-[5-[1,3-Dihydro-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino]-6-oxohexyl]3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium inner salt
Purity:	≥95% (HPLC)
Molecular Weight:	775.97

View Full Technical Details for Cyanine 5 Tyramide

Technical Data
Citations

Biological Activity for Cyanine 5 Tyramide

Cyanine 5 Tyramide is a red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data for Cyanine 5 Tyramide

Emission Color	Red
λ_abs	651 nm
λ_em	665 nm
Extinction Coefficient (ε)	250000 M^-1cm^-1
Closest Laser line	647 nm
Application	Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Cyanine 5 Tyramide

M. Wt	775.97
Formula	C₄₁H₄₉N₃O₈S₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	1431148-26-3
PubChem ID	135290897
InChi Key	NHSJCIBSNKLAMA-UHFFFAOYSA-N
Smiles	CC[N+]1=C(C(C)(C2=CC(S([O-])(=O)=O)=CC=C12)C)/C=C/C=C/C=C3N(C4=CC=C(S(O)(=O)=O)C=C4C/3(C)C)CCCCCC(NCCC5=CC=C(C=C5)O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Cyanine 5 Tyramide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	11.64	15

Preparing Stock Solutions for Cyanine 5 Tyramide

The following data is based on the product molecular weight 775.97.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.15 mM	8.59 mL	42.96 mL	85.91 mL
0.75 mM	1.72 mL	8.59 mL	17.18 mL
1.5 mM	0.86 mL	4.30 mL	8.59 mL
7.5 mM	0.17 mL	0.86 mL	1.72 mL

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References for Cyanine 5 Tyramide

References are publications that support the biological activity of the product.

Matsumoto Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer. Br.J.Cancer 2017 PMID: 28881356
Wong Identification of differentially expressed genes from ovarian cancer cells by MICROMAX cDNA microarray system. Biotechniques 2001 PMID: 11252802
Chanda Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer. PLoS One 2017 PMID: 28493978