FFN 200 dihydrochloride

Name: FFN 200 dihydrochloride
Brand: Tocris Bioscience
SKU: 5911

Tocris Bioscience, part of Bio-Techne | Catalog # 5911

Citations (2)

COA / SDS

Selective fluorescent VMAT2 substrate

Key Product Details

Description:	Selective fluorescent VMAT2 substrate
Chemical Name:	4-(2-Aminoethyl)-7-amino-2H-1-benzopyran-2-one dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	277.15

View Full Technical Details for FFN 200 dihydrochloride

Technical Data
Citations

Biological Activity for FFN 200 dihydrochloride

FFN 200 dihydrochloride is a selective fluorescent VMAT2 substrate. Selectively traces exocytosis in dopaminergic neuronal cell culture and brain tissue. Excitation and emission maxima are 352 and 451 nm, respectively.

Licensing Information

Sold with the permission of Columbia University.

Applications in Cultured Dopamine Neurons

FFN200 in vesicle clusters in axons of cultured dopaminergic neurons, and exocytosis upon high potassium stimulation (time lapse acquisition at 12 images/min).

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Technical Data for FFN 200 dihydrochloride

M. Wt	277.15
Formula	C₁₁H₁₂N₂O₂.2HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2080306-27-8
PubChem ID	124201850
InChi Key	YNQXRSNAOWBGLD-UHFFFAOYSA-N
Smiles	NC1=CC(OC(C=C2CCN)=O)=C2C=C1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for FFN 200 dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	27.71	100
	DMSO	27.71	100

Preparing Stock Solutions for FFN 200 dihydrochloride

The following data is based on the product molecular weight 277.15.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.61 mL	18.04 mL	36.08 mL
5 mM	0.72 mL	3.61 mL	7.22 mL
10 mM	0.36 mL	1.80 mL	3.61 mL
50 mM	0.07 mL	0.36 mL	0.72 mL

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References for FFN 200 dihydrochloride

References are publications that support the biological activity of the product.

Li Probes for monitoring regulated exocytosis. Cell Calcium 2017 PMID: 28089267
Pereira Fluorescent false neurotransmitter reveals functionally silent DA vesicle clusters in the striatum. Nat.Neurosci. 2015 PMID: 26900925