FITC-labelled Keap1-Nrf2 probe

Name: FITC-labelled Keap1-Nrf2 probe
Brand: Tocris Bioscience
SKU: 7627

Tocris Bioscience, part of Bio-Techne | Catalog # 7627

COA / SDS

Fluorescent Keap1-Nrf2 peptide

Discontinued Product

7627 has been discontinued. View all Fluorescent Probes products.

Key Product Details

Description:	Fluorescent Keap1-Nrf2 peptide
Purity:	≥90% (HPLC)
Molecular Weight:	1440.5

View Full Technical Details for FITC-labelled Keap1-Nrf2 probe

Technical Data
Citations

Biological Activity for FITC-labelled Keap1-Nrf2 probe

FITC-labelled Keap1-Nrf2 probe is a fluorescent Keap1-Nrf2 peptide (K_d = 22.3 nM). It can be used to assess the binding affinities of Nrf2 peptide inhibitors in Fluorescence Polarization (FP) displacement assays and TR-FRET.

Optical Data for FITC-labelled Keap1-Nrf2 probe

Emission Color	Green
λ_abs	495 nm
λ_em	525 nm
Closest Laser line	488 nm
Application	Fluorescence polarization (FP) displacement assays, TR-FRET

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Technical Data for FITC-labelled Keap1-Nrf2 probe

M. Wt	1440.5
Formula	C₆₇H₈₁N₁₁O₂₃S
Single Letter Sequence	LDEETGEFL (Modifications: Leu-1 = FITC-Leu, Leu-9 = C-terminal amide)
Storage	Store at -20°C
Purity	≥90% (HPLC)
InChi Key	RDECLUWFJCNNAY-NZNAQQBOSA-N
Smiles	O=C(N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H]([C@H](O)C)C(NCC(N[C@@H](CCC(O)=O)C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CC(C)C)C(N)=O)=O)=O)=O)=O)=O)=O)=O)[C@@H](NC(NC(C=C2C(O)=O)=CC=C2C(C3=C4C=C(O)C=C3)=C(C=C5)C(O4)=CC5=O)=S)CC(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for FITC-labelled Keap1-Nrf2 probe

References are publications that support the biological activity of the product.

Inoyama Optimization of fluorescently labeled Nrf2 peptide probes and the development of a fluorescence polarization assay for the discovery of inhibitors of Keap1-Nrf2 interaction. J.Biomol.Screen 2012 PMID: 22156223
Wang Development of an enzyme-linked immunosorbent assay for Keap1-Nrf2 interaction inhibitors identification. Redox Biol. 2020 PMID: 32422542
Chen Kinetic analyses of Keap1-Nrf2 interaction and determination of the minimal Nrf2 peptide sequence required for Keap1 binding using surface plasmon resonance. Chem.Biol.Drug Des. 2011 PMID: 21920027