FITC
Tocris Bioscience, part of Bio-Techne | Catalog # 5440
Key Product Details
| Description: | Green fluorescent dye |
| Alternative Names: | Fluorescein-5-Isothiocyanate,FITC Isomer 1 |
| Chemical Name: | 3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 389.38 |
Biological Activity for FITC
Key information: FITC (an amine-reactive version of the parent dye, fluorescein) is a widely-used green fluorescent dye supplied with an isothiocyanate reactive group for the labeling of primary amines. FITC is bright and has good water-solubility, however it is sensitive to changes in pH and temperature, and has a relatively high photobleaching rate.
Application: Suitable for flow cytometry, immunofluorescence (IHC, ICC), fluorescence microscopy, fluorescence in situ hybridization (FISH), biochemical assays.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 495 nm and 525 nm, respectively; quantum yield = 0.92; extinction coefficient = 75,000 M-1cm-1; A280 = 0.35.
Optical Data for FITC
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 495 nm |
| λem | 525 nm |
| Extinction Coefficient (ε) | 75000 M-1cm-1 |
| Quantum Yield (φ) | 0.92 |
| Closest Laser line | 488 nm |
| Reactive Group | Isothiocyanate |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.35 |
| Application | Flow Cytometry, Immunofluorescence (IHC, ICC), Fluorescence Microscopy, FISH |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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