Fluorescein Tyramide

Name: Fluorescein Tyramide
Brand: Tocris Bioscience
SKU: 6456

Tocris Bioscience, part of Bio-Techne | Catalog # 6456

Citations (1)

COA / SDS

Green fluorescent reagent widely used for signal amplification in IHC and FISH

Key Product Details

Description:	Green fluorescent reagent widely used for signal amplification in IHC and FISH
Chemical Name:	3',6'-Dihydroxy-N-(4-hydroxyphenylethyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(6)-carboxamide
Purity:	≥95% (HPLC)
Molecular Weight:	495.48

View Full Technical Details for Fluorescein Tyramide

Technical Data
Citations

Biological Activity for Fluorescein Tyramide

Fluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data for Fluorescein Tyramide

Emission Color	Green
λ_abs	494 nm
λ_em	517 nm
Extinction Coefficient (ε)	70000 M^-1cm^-1
Closest Laser line	488 nm
Application	Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Fluorescein Tyramide

M. Wt	495.48
Formula	C₂₉H₂₁NO₇
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	210236-90-1
InChi Key	MJQMGAFVBOOBIB-UHFFFAOYSA-N
Smiles	OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)C5=CC=C(C(NCCC6=CC=C(C=C6)O)=O)C=C5C(O3)=O)C=C1.OC7=CC8=C(C9(C%10=CC=C(C=C%10O8)O)C%11=CC(C(NCCC%12=CC=C(C=C%12)O)=O)=CC=C%11C(O9)=O)C=C7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Fluorescein Tyramide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	11.89	24

Preparing Stock Solutions for Fluorescein Tyramide

The following data is based on the product molecular weight 495.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.24 mM	8.41 mL	42.05 mL	84.09 mL
1.2 mM	1.68 mL	8.41 mL	16.82 mL
2.4 mM	0.84 mL	4.20 mL	8.41 mL
12 mM	0.17 mL	0.84 mL	1.68 mL

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Reconstitution Calculator

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References for Fluorescein Tyramide

References are publications that support the biological activity of the product.

Bobrow Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 1989 PMID: 2558138
van Gijlswijk Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 1997 PMID: 9071319
Schriml Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 1999 PMID: 10489619
Hopman Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 1998 PMID: 9603790
Karkmann Enzymatic signal amplification for sensitive detection of intracellular antigens by flow cytometry. J.Immunol.Methods 1999 PMID: 10594358